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After the events defeating Electro and Green Goblin. (Y/N) Parker lose his love one that he didn't save her in time... Now he have to move on as New York's protector... One day... He meet the mystery girl name Huijin Park invited him the secret society of Human Hybrids with Bugs called Nest.

But.... There is the evil threats coming is the Pet Shop and his former childhood friend form the evil team known as Sinister Six. Will Spider-Man will stop him or not?